The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis.

Arthrinium phaeospermum is the major pathogen responsible for the significant stem disease "blight" in B. pervariabilis × D. grandis. The interacting proteins of the key pathogenic factor ApCtf1ß, BDUbc and BDSKL1, have previously been obtained by two-hybrid, BiFC, GST pull-down yeast assays. However, the functions of these interacting proteins remain unknown. This study successfully obtained transgenic plants overexpressing BDUbc, BDSKL1, and BDUbc + BDSKL1 via Agrobacterium-mediated gene overexpression. qRT-PCR analysis revealed significantly increased expression levels of BDUbc and BDSKL1 in the transgenic plants. After infection with the pathogenic spore suspension, the disease incidence and severity index significantly decreased across all three transgenic plants, accompanied by a marked increase in defense enzyme levels. Notably, the co-transformed plant, OE-BDUbc + BDSKL1, demonstrated the lowest disease incidence and severity index among the transgenic variants. These results not only indicate that BDUbc and BDSKL1 are disease-resistant genes, but also that these two genes may exhibit a synergistic enhancement effect, which further improves the resistance to blight in Bambusa pervariabilis × Dendrocalamopsis grandis.

In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems.

The low multiplication and ex vitro survival rates during acclimatization in the culture house limit the in vitro mass propagation of B. vulgaris. Several scientific studies have described the development of different protocols for bamboo; however, not all of them address the effects of these systems on plant morphology, physiology, and biochemistry in vitro. In this chapter, a complete and optimized protocol is described for plants propagated via organogenesis in temporary immersion systems. In addition, the morphophysiological and biochemical characterization of the plants as well as the survival rates of the obtained plants under ex vitro conditions are analyzed. The obtained results will be the basis for the development of a technology for in vitro propagation as an alternative for the production of plants of the species.

Comparative analysis of endophytic fungal communities in bamboo species Phyllostachys edulis, Bambusa rigida, and Pleioblastus amarus.

Fungal endophytes in plant leaf mesophyll form mutually beneficial associations through carbon assimilation, synthesis of biologically active chemicals, and enhancement of aesthetic and nutritional value. Here, we compared community structure, diversity, and richness of endophytic fungi in the leaves of three bamboo species, including Phyllostachys edulis (MZ), Bambusa rigida (KZ), and Pleioblastus amarus (YT) via high-throughput Illumina sequencing. In total, 1070 operational taxonomic units (OTUs) were retrieved and classified into 7 phylum, 27 classes, 82 orders, 185 families, 310 genus, and 448 species. Dominant genera were Cladosporium, Trichomerium, Hannaella, Ascomycota, Sporobolomyces, Camptophora and Strelitziana. The highest fungal diversity was observed in Pleioblastus amarus, followed by Bambusa rigida, and Phyllostachys edulis. Comparatively, monopodial species Ph. edulis and sympodial B. rigida, mixed P. amarus revealed the highest richness of endophytic fungi. We retrieved a few biocontrol agents, Sarocladium and Paraconiothyrium, and unique Sporobolomyces, Camptophora, and Strelitziana genera. FUNGuild analysis revealed the surrounding environment (The annual average temperature is between 15 and 25 °C, and the relative humidity of the air is above 83% all year round) as a source of fungal accumulation in bamboo leaves and their pathogenic nature. Our results provide precise knowledge for better managing bamboo forests and pave the way for isolating secondary metabolites and potential bioactive compounds.

Mechanical superiority of Pseudoxytenanthera bamboo for sustainable engineering solutions.

The advancement in natural fibre composites has replaced synthetic fibres in various commercial sectors. Bamboo species possess high mechanical properties due to their lignocellulosic fibre content, which makes them suitable for engineering applications and potential alternatives to solid wood. However, despite Bamboo being composed of 130 genera and 1700 different species, out of which many still remains underexplored. In this study, we investigated the, Lignocellulosic profiling, fibre strength, and mechanical characterization of two species of Pseudoxytenanthera Bamboo: Pseudoxytenanthera ritchiei, Pseudopxytenanthera stocksii, and the results obtained were compared with Bambusa balcooa, one of the priority species of bamboo identified by The International Plant Genetic Resources Institute (IPGRI). BET (Brunauer-Emmett-Teller) was used to quantify the samples' density, while SEM-EDX and FTIR spectroscopy were used for elemental analysis. The samples were then subjected to tensile test in addition, thermogravimetric analysis and water absorption test were carried out for the three species. The results showed that Pseudoxytenanthera species possessed superior chemical and mechanical characteristics compared to the priority species of bamboo used for composites. Out of the two Pseudoxytenanthera species studied, Pseudoxytenanthera stocksii exhibited the highest values of cellulose, hemicellulose, lignin, pectin, ash, carbon, and silicon, indicating its chemical superiority. Moreover, Pseudoxytenanthera stocksii also showed higher mechanical values for tensile strength, making it suitable for a variety of engineering applications. The TGA values also indicated that Pseudoxytenanthera stocksii is stable at high temperatures when compared with other natural fibres.

Transcriptomic and metabolomic analyses reveal the flavor of bitterness in the tip shoots of Bambusa oldhamii Munro.

The young bamboo shoot of Bambusa oldhamii (green bamboo) has a good taste and is rich in nutrition and widely used in traditional Chinese cuisines. But the shoots flavor of Bambusa oldhamii changed from deliciously sweet to a little bitter when the shoots grew from underground to aboveground. In this paper, we explored the bitterness chemicals of the green bamboo shoot when growing from underground to aboveground using transcriptome and metabolome techniques. Finally, several bitter chemicals were mined out counting for the flavor transformation, such as Solanidine, Amygdalin, Salicin, Arbutin, and others. The transcription factor family of AP2/ERF plays the main role in key bitter chemical regulation via correlation analysis. Moreover, the pathway of Biosynthesis of phenylpropanoids might be the key pathway in the formation of the bitter chemicals in green bamboo shoot development.

Identification and Characterization of the BBX Gene Family in Bambusa pervariabilis × Dendrocalamopsis grandis and Their Potential Role under Adverse Environmental Stresses.

Zinc finger protein (ZFP) transcription factors play a pivotal role in regulating plant growth, development, and response to biotic and abiotic stresses. Although extensively characterized in model organisms, these genes have yet to be reported in bamboo plants, and their expression information is lacking. Therefore, we identified 21 B-box (BBX) genes from a transcriptome analysis of Bambusa pervariabilis × Dendrocalamopsis grandis. Consequently, multiple sequence alignments and an analysis of conserved motifs showed that they all had highly similar structures. The BBX genes were divided into four subgroups according to their phylogenetic relationships and conserved domains. A GO analysis predicted multiple functions of the BBX genes in photomorphogenesis, metabolic processes, and biological regulation. We assessed the expression profiles of 21 BBX genes via qRT-PCR under different adversity conditions. Among them, eight genes were significantly up-regulated under water deficit stress (BBX4, BBX10, BBX11, BBX14, BBX15, BBX16, BBX17, and BBX21), nine under salt stress (BBX2, BBX3, BBX7, BBX9, BBX10, BBX12, BBX15, BBX16, and BBX21), twelve under cold stress (BBX1, BBX2, BBX4, BBX7, BBX10, BBX12, BBX14, BBX15, BBX17, BBX18, BBX19, and BBX21), and twelve under pathogen infestation stress (BBX1, BBX2, BBX4, BBX7, BBX10, BBX12, BBX14, BBX15, BBX17, BBX18, BBX19, and BBX21). Three genes (BBX10, BBX15, and BBX21) were significantly up-regulated under both biotic and abiotic stresses. These results suggest that the BBX gene family is integral to plant growth, development, and response to multivariate stresses. In conclusion, we have comprehensively analyzed the BDBBX genes under various adversity stress conditions, thus providing valuable information for further functional studies of this gene family.

Unraveling the Lignin Structural Variation in Different Bamboo Species.

The structure of cellulolytic enzyme lignin (CEL) prepared from three bamboo species (Neosinocalamus affinis, Bambusa lapidea, and Dendrocalamus brandisii) has been characterized by different analytical methods. The chemical composition analysis revealed a higher lignin content, up to 32.6% of B. lapidea as compared to that of N. affinis (20.7%) and D. brandisii (23.8%). The results indicated that bamboo lignin was a p-hydroxyphenyl-guaiacyl-syringyl (H-G-S) lignin associated with p-coumarates and ferulates. Advanced NMR analyses displayed that the isolated CELs were extensively acylated at the γ-carbon of the lignin side chain (with either acetate and/or p-coumarate groups). Moreover, a predominance of S over G lignin moieties was found in CELs of N. affinis and B. lapidea, with the lowest S/G ratio observed in D. brandisii lignin. Catalytic hydrogenolysis of lignin demonstrated that 4-propyl-substituted syringol/guaiacol and propanol guaiacol/syringol derived from ß-O-4' moieties, and methyl coumarate/ferulate derived from hydroxycinnamic units were identified as the six major monomeric products. We anticipate that the insights of this work could shed light on the sufficient understanding of lignin, which could open a new avenue to facilitate the efficient utilization of bamboo.

Antimicrobial activity and chemical profile of wood vinegar from eucalyptus (Eucalyptus urophylla x Eucalyptus grandis - clone I144) and bamboo (Bambusa vulgaris).

Microbial resistance to drugs is a public health problem; therefore, there is a search for alternatives to replace conventional products with natural agents. One of the potential antimicrobial agents is wood vinegar derived from the carbonization of lignocellulosic raw materials. The objectives of the present work were to evaluate the antibacterial and antifungal action of two kinds of wood vinegar (WV), one of Eucalyptus urograndis wood and another of Bambusa vulgaris biomass, and determine their chemical profile. The antimicrobial effect was assessed against Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enteritidis, Escherichia coli, Streptococcus agalactiae, and Candida albicans. The minimum inhibitory concentration and the minimum bactericidal and fungicidal concentrations were determined. Micrographs of the microorganisms before and after exposure to both kinds of wood vinegar were obtained by scanning electron microscopy. The chemical profile of the eucalyptus and bamboo vinegar was carried out by gas chromatography and mass spectrometry (GC/MS). Both types of WV presented significant antimicrobial activity, with the bamboo one having a higher efficiency. Both studied pyroligneous extracts seem promising for developing natural antimicrobials due to their efficiency against pathogens. GC/MS analyses demonstrated that the chemical profiles of both kinds of WV were similar but with some significant differences. The major component of the eucalyptus vinegar was furfural (17.2%), while the bamboo WV was phenol (15.3%). Several compounds in both WVs have proven antimicrobial activity, such as acetic acid, furfural, phenol, cresols, guaiacol, and xylenols. Together, they are the major in the chemical composition of the organic fraction of both WVs. Bamboo vinegar had a more expressive content of organic acids. Micrographs of microorganisms taken after exposure to both kinds of wood vinegar displayed several cell modifications. The potential of both types of wood vinegar as a basis for natural antimicrobial products seems feasible due to their proven effect on inhibiting the microorganisms' growth assessed in this experiment.

Sustainable technology: potential of biomass (Bambusa tuldoides) for biological denitrification of wastewater generated in shrimp farming.

Wastewater from shrimp farming is rich in organic material, solids, and nutrients, which cause a series of environmental problems when released into the environment. Currently, for the removal of nitrogen compounds from wastewater, among the most studied methods is biological denitrification. The objective of this study was to evaluate the operational parameters for the development of a more sustainable technology for the removal of nitrogen compounds from shrimp farm wastewater, using Bambusa tuldoides (a species of bamboo) as a source of carbon and a material conducive to the development of selected denitrifying bacteria. To optimize the process, biological denitrification assays were performed varying the following parameters: bamboo length (cm), pH, temperature, and stoichiometric proportions of C and N. The operational stability of the process with the reuse of the bamboo biomass was also evaluated. Cronobacter sakazakii and Bacillus cereus were identified as denitrifying microorganisms present in reactor with bamboo biomass. The best operational conditions observed were pH 6 to 7 and temperature 30 to 35 °C, and the addition of an external carbon source was not necessary for the denitrification process to occur efficiently. Under these conditions, biological denitrification occurred with an average efficiency above 90% based on the removal of the nitrogen contaminants evaluated (NO3-N and NO2-N). Regarding operational stability, 8 cycles were performed using the same source of carbon without reducing the efficiency of the process.

Verification of the Interaction Target Protein of the Effector ApCE22 of Arthrinium phaeospermum in Bambusa pervariabilis × Dendrocalamopsis grandis.

The study of interaction proteins of the pathogen A. phaeospermum effector protein is an important means to analyze the disease-resistance mechanism of Bambusa pervariabilis × Dendrocalamopsis grandis shoot blight. To obtain the proteins interacting with the effector ApCE22 of A. phaeospermum, 27 proteins interacting with the effector ApCE22 were initially identified via a yeast two-hybrid assay, of which four interaction proteins were obtained after one-to-one validation. The B2 protein and the chaperone protein DnaJ chloroplast protein were then verified to interact with the ApCE22 effector protein by bimolecular fluorescence complementation and GST pull-down methods. Advanced structure prediction showed that the B2 protein contained the DCD functional domain related to plant development and cell death, and the DnaJ protein contained the DnaJ domain related to stress resistance. The results showed that both the B2 protein and DnaJ protein in B. pervariabilis × D. grandis were the target interaction proteins of the ApCE22 effector of A. phaeospermum and related to the stress resistance of the host B. pervariabilis × D. grandis. The successful identification of the pathogen effector interaction target protein in B. pervariabilis × D. grandis plays an important role in the mechanism of pathogen-host interaction, thus providing a theoretical basis for the control of B. pervariabilis × D. grandis shoot blight.

Geographic and longitudinal variations of anatomical characteristics and mechanical properties in three bamboo species naturally grown in Lombok Island, Indonesia.

The anatomical characteristics (fiber length and fiber area) and mechanical properties (modulus of elasticity, modulus of rupture, compressive strength, tensile Young's modulus, and tensile strength) of Bambusa vulgaris, Bambusa maculata, and Gigantochloa atter, naturally growing at four different sites in Lombok Island, Indonesia, were examined for evaluating geographic and longitudinal variations by mixed-effects modeling to effectively utilize bamboo culm resources for structural materials. We found geographic and longitudinal variations of bamboo culm properties in these three species. Based on the results, we concluded that, for utilization of bamboo culm as a structural material, variation of individual culm rather than site, and longitudinal variations should be considered for Bambusa species and G. atter, respectively.

Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes.

OBJECTIVES: There is evidence that mitochondrial dysfunction mediated by hyperglycemia increases the incidence of diabetes and age-related insulin resistance. Thus, maintaining mitochondrial integrity may provide alternative therapeutic approach in diabetes treatment. This study aimed to evaluate the effect of Bambusa vulgaris leaf extract on mitochondrial biogenesis in the pancreas of diabetic rats. METHODS: 11 weeks old male rats (n=30) were purchased, and sorted into the following groups: control, diabetic control, diabetes + metformin (100 mg/kg), diabetes + Aq. B. vulgaris (100 mg/kg), diabetes + Aq. B. vulgaris (200 mg/kg), and diabetes + Aq. B. vulgaris (300 mg/kg). Diabetes was induced in the rats by a single dose of 65 mg/kg streptozotocin (STZ). The mRNA expression of genes related to mitochondria biogenesis (pgc-1α, Nrf2, GSK3ß, AMPK and SIRT2) and genes of Nrf2-Keap1-ARE signaling pathway were determined by reverse transcriptase polymerase chain reaction. Molecular docking studies including lock and key docking and prime MM-GBSA were incorporated to identify the lead chemical compounds in Bambusa vulgari. RESULTS: The results showed that B. vulgaris leaf extract promotes mitochondrial biogenesis via altering the mRNA expression of mitochondrial master regulator pgc-1α, other upstream genes, and the Nrf2-Keap1-ARE antioxidant pathway. Through molecular docking results, cryptochlorogenic acid, hesperidin, orientin, vitexin, scopolin, and neochlorogenic were found as the crucial chemicals in B. vulgaris with the most modulating effect on PGC-1α, AMPK, and GSK3. CONCLUSIONS: This study thus suggests that B. vulgaris leaf extract restores the integrity of mitochondria in diabetic rats.

Exploring the promising potential of fallen bamboo leaves (Bambusa bambos) for efficient removal of crystal violet from wastewater.

Fallen bamboo leaves (Bambusa bambos), hereinafter BL have been designed to be transformed into an efficient and sustainable adsorbent for the removal of crystal violet (CV) dye from wastewater with up to 95% scavenging ability. BL have been characterized by Fourier transform infrared (FTIR) spectra, field emission scanning electron microscopy (FESEM), and zero point charge (pHzpc). The maximum adsorption capacity is 30 mg/g at pH 10. Physico-chemical parameters have been investigated concerning pH, contact time, initial concentration, and coexistent ions. Pseudo-second-order kinetics is followed best (R2 =0.999) signifying a chemisorption pathway. Besides, intra-particle diffusion plays a governing role in the film diffusion of crystal violet into the core of the adsorbent. Langmuir isotherm model fits best (R2=0.972) suggesting a uniform, monolayer, and homogeneous adsorption. Regeneration was successful with methanol (65%) and reusability was tested for three cycles and was found to retain activity up to 80%. Analysis of CV containing industrial effluent suggests that a 36.8% reduction is possible with BL. The effect of co-existent ions suggests little influence on the adsorption. Compared to other contemporary and relevant adsorbents, it can be concluded that BL can be exercised for the sustainable decontamination of CV-containing wastewater.

Bambusa bambos, the giant thorny bamboo is an abundantly available plant throughout the year, has been successfully exercised using its fallen leaves to scavenge crystal violet, a cationic dye from water and wastewater. Up to 95% adsorption was noticed at ambient conditions, which when further extrapolated for industrial effluent analysis, shows a remarkable 36.8% decontamination/cycle. With an adsorption capacity of 30 mg/g, it enjoys an edge over contemporary phytosorbents. The process is free from any chemical treatment, green in nature, and sustainable. Abundant availability and economic viability allow an impactful application of fallen bamboo leaves for water and wastewater treatment in a lab-to-land sequence.

Coleoborers in Bambusa sp. in the Southern Amazon / Coleobrocas em Bambusa sp. na Amazonia Meridional

Abstract Bamboo (Bambusa sp.) is a grass species with high potential for exploitation, however, this raw material is easily attacked by xylophagous agents such as Coleoptera. The objective of this study is thus to analyse the presence of wood-boring beetles associated with Bambusa sp. in Alta Floresta, Mato Grosso, Brazil. The study was carried out in a bamboo plantation, from August 2016 to July 2017. Two models of ethanolic traps were used, PET Santa Maria and ESALQ-84. Three families of wood-boring beetles were observed in this study: Bostrichidae, Cerambycidae and Curculionidae. 2,144 individuals were identified, belonging to 19 species, in four subfamilies and 11 tribes, and the species Xyleborus affinis was the most frequently collected. Thus, this study constitutes an important contribution to the knowledge of coleoborers associated with bamboo plantations, with the registration of the occurrence of species, as well as contributing to the knowledge of the entomofauna associated with species cultivated in the Southern Amazon.

Resumo O bambu (Bambusa sp.) é uma espécie de gramínea com alto potencial de exploração de fibras, no entanto, essa matéria-prima é facilmente atacada por agentes xilófagos, como os coleópteros. O objetivo deste estudo foi analisar a presença de besouros associados a Bambusa sp. em Alta Floresta, Mato Grosso, Brasil. O estudo foi realizado em um plantio de bambu, no período de agosto de 2016 a julho de 2017. Foram utilizados dois modelos de armadilhas etanólicas, o PET Santa Maria e ESALQ-84. Três famílias de coleobrocas foram observadas neste estudo: Bostrichidae, Cerambycidae e Curculionidae. Foram identificados 2,144 indivíduos, pertencentes a 19 espécies, em quatro subfamílias e 11 tribos, e Xyleborus affinis foi a espécie com maior número de indivíduos coletados. Assim, este estudo constitui-se em uma importante contribuição para o conhecimento de coleobrocas associadas a plantios de bamboo, com o registro de ocorrência de espécies, bem como contribui para o conhecimento da entomofauna associada a espécies cultivadas na Amazônia Meridional.

A new species of Discochiton Hodgson & Williams (Hemiptera: Coccomorpha: Coccidae) on bamboo from Myanmar.

The adult female and first-instar nymph of a new species of Discochiton Hodgson & Williams, 2018, Discochiton bambusae Choi & Lee sp. n. are described and illustrated. They were collected on the culms of Bambusa tulda (Poaceae: Bambusoideae) in Myanmar. The adult female has several unique morphological features, such as a large body, up to 15 mm long; legs much reduced; and sclerotized plates forming submedial bands or patches on the venter. Furthermore, the adult female has more marginal radial lines and stigmatic spines than are found in other Discochiton species. The first-instar nymph is also much larger than those of other Discochiton species, and is also distinct in having tibial setae, and a greater number of spiracular disc-pores and marginal setae compared to those of other species of Discochiton.

Review and new data on the aphid fauna (Hemiptera, Aphidoidea) of the Socialist Republic of Vietnam with a description of a new genus and a new species of the family Hormaphididae.

An up-to-date information on aphid fauna (Hemiptera, Aphidoidea) of Vietnam based on literature data and original material is presented. The updated check-list of aphids includes 47 species, and also 15 species identified only to genus level. Protastegopteryx vietnamensis gen. nov., sp. nov. from Bambusa sp., collected in Ct Tin National Park (Dong Nai Province), is described. The species is illustrated and its biometric data is provided. The described aphid genus is closely related to Astegopteryx Karsch, 1890. The taxonomic position of the genus is discussed.

Coleoborers in Bambusa sp. in the Southern Amazon / Coleobrocas em Bambusa sp. na Amazonia Meridional

Bamboo (Bambusa sp.) is a grass species with high potential for exploitation, however, this raw material is easily attacked by xylophagous agents such as Coleoptera. The objective of this study is thus to analyse the presence of wood-boring beetles associated with Bambusa sp. in Alta Floresta, Mato Grosso, Brazil. The study was carried out in a bamboo plantation, from August 2016 to July 2017. Two models of ethanolic traps were used, PET Santa Maria and ESALQ-84. Three families of wood-boring beetles were observed in this study: Bostrichidae, Cerambycidae and Curculionidae. 2,144 individuals were identified, belonging to 19 species, in four subfamilies and 11 tribes, and the species Xyleborus affinis was the most frequently collected. Thus, this study constitutes an important contribution to the knowledge of coleoborers associated with bamboo plantations, with the registration of the occurrence of species, as well as contributing to the knowledge of the entomofauna associated with species cultivated in the Southern Amazon.

O bambu (Bambusa sp.) é uma espécie de gramínea com alto potencial de exploração de fibras, no entanto, essa matéria-prima é facilmente atacada por agentes xilófagos, como os coleópteros. O objetivo deste estudo foi analisar a presença de besouros associados a Bambusa sp. em Alta Floresta, Mato Grosso, Brasil. O estudo foi realizado em um plantio de bambu, no período de agosto de 2016 a julho de 2017. Foram utilizados dois modelos de armadilhas etanólicas, o PET Santa Maria e ESALQ-84. Três famílias de coleobrocas foram observadas neste estudo: Bostrichidae, Cerambycidae e Curculionidae. Foram identificados 2,144 indivíduos, pertencentes a 19 espécies, em quatro subfamílias e 11 tribos, e Xyleborus affinis foi a espécie com maior número de indivíduos coletados. Assim, este estudo constitui-se em uma importante contribuição para o conhecimento de coleobrocas associadas a plantios de bamboo, com o registro de ocorrência de espécies, bem como contribui para o conhecimento da entomofauna associada a espécies cultivadas na Amazônia Meridional.

Physico-thermal and emission properties of tissue cultured clone from Bambusa balcoaa (Beema bamboo) and Oxytenanthera abyssinica as sustainable solid biofuels.

In the search for alternatives to wood fuel, to meet the bio-energy requirement of an ever-increasing global population, the International Network for Bamboo and Rattan has supported farmers in many tropical countries to establish plantations of Beema bamboo (a tissue-cultured clone from Bambusa vulgaris) and Oxytenanthera abyssinica for bio-energy production. The quality of these species as solid biofuels is unknown due to the absence of data on their physico-thermal and emission characteristics. Using the American Standard for Testing and Materials and other internationally accepted standards, their ultimate and proximate analysis, and physico-thermal and emission properties were evaluated. Beema bamboo and O. abyssinica have high Hydrogen, organic and fixed Carbon contents and low quantities of ash, moisture content, volatile matter, Oxygen, Nitrogen and Sulphur. This will contribute to their heating values and low oxide emissions. Based on their High Heating Values (Beema bamboo = 23.22 MJ/kg; O. abyssinica = 23.26 MJ/kg), the species will be suitable for high energy-using applications. The Particulate Matter and Carbon Monoxide concentrations (Beema bamboo: 90 ug/m3 and 2.83 ppm respectively; O. abyssinica: 77.33 ug/m3 and 3.20 ppm respectively) are lower than the threshold (35000 ug/m3 and 9 ppm respectively) approved by the United States Environmental Protection Agency. These properties make the species good raw materials for solid biofuel which is safe for indoor use. Their use will contribute to reducing pressure on tropical forests for wood fuel and the health hazards associated with fossil fuel use.

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight.

The shoot blight of Bambusa pervariabilis × Dendrocalamopsis grandis caused by Arthrinium phaeospermum made bamboo die in a large area, resulting in serious ecological and economic losses. Dual RNA-seq was used to sequence and analyze the transcriptome data of A. phaeospermum and B. pervariabilis × D. grandis in the four periods after the pathogen infected the host and to screen the candidate effectors of the pathogen related to the infection. After the identification of the effectors by the tobacco transient expression system, the functions of these effectors were verified by gene knockout. Fifty-three differentially expressed candidate effectors were obtained by differential gene expression analysis and effector prediction. Among them, the effectors ApCE12 and ApCE22 can cause programmed cell death in tobacco. The disease index of B. pervariabilis × D. grandis inoculated with mutant ΔApCE12 and mutant ΔApCE22 strains were 52.5% and 47.5%, respectively, which was significantly lower than that of the wild-type strains (80%), the ApCE12 complementary strain (77.5%), and the ApCE22 complementary strain (75%). The tolerance of the mutant ΔApCE12 and mutant ΔApCE22 strains to H2O2 and NaCl stress was significantly lower than that of the wild-type strain and the ApCE12 complementary and ApCE22 complementary strains, but there was no difference in their tolerance to Congo red. Therefore, this study shows that the effectors ApCE12 and ApCE22 play an important role in A. phaeospermum virulence and response to H2O2 and NaCl stress.

Bambusaecoccus maolanensis, a new soft scale genus and species from China (Hemiptera: Coccomorpha: Coccidae).

A new genus of soft scale insect from Guizhou Province, China, Bambusaecoccus Meng Xing gen. n., with Bambusaecoccus maolanensis Meng Xing sp. n. (Hemiptera: Coccidae: Paralecaniini) as type species, is described and illustrated. The species was collected from bamboo, Bambusa (Dendrocalamopsis) sp. (Poaceae: Bambusoideae), under carton shelters built by the ant Crematogaster subnuda nigrosubnuda zdikmen 2010 (Hymenoptera: Formicidae: Myrmicinae). Type specimens of the new species are deposited at the Institute of Entomology, Guizhou University, Guiyang, China (GUGC).